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AcceGen Biotechnology bv2 microglia
Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in <t>BV2</t> cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.
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Vector Laboratories abc ap kit
Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in <t>BV2</t> cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.
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Vector Laboratories vectastain elite abc hrp reagent
Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in <t>BV2</t> cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.
Vectastain Elite Abc Hrp Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology immunocruz rabbit abc staining system
Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in <t>BV2</t> cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.
Immunocruz Rabbit Abc Staining System, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories vector laboratory kit
Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in <t>BV2</t> cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.
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Santa Cruz Biotechnology immunocruz goat bc staining system sc 2023
Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in <t>BV2</t> cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.
Immunocruz Goat Bc Staining System Sc 2023, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AMS Biotechnology proteus vulgaris
Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in <t>BV2</t> cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.
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Vector Laboratories vectastain elite abc kit universal
Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in <t>BV2</t> cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.
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Image Search Results


Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in BV2 cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.

Journal: Life

Article Title: In Vitro Antioxidant and Anti-Neuroinflammatory Effects of Elsholtzia blanda Benth

doi: 10.3390/life15060983

Figure Lengend Snippet: Figure 4. Effects of EBB on LPS-induced protein expression of iNOS, COX-2, and mitogen-activated protein kinase (MAPK) phosphorylation in BV2 cells. Cells were pretreated with different concen- trations of EBB for 1 h before treatment with 0.2 µg/mL LPS. After incubation for 24 h, the iNOS and COX-2 protein levels were measured by Western blotting (a,b). After incubation for 15 min, the MAPK phosphorylation and non-phosphorylation levels were analyzed (c,d). The band intensity was analyzed by ImageJ program (Version 1.54p). Each column represents mean ± SEM (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. VH, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.

Article Snippet: BV2 microglia were purchased from AcceGen (Fairfield, NJ, USA).

Techniques: Expressing, Phospho-proteomics, Incubation, Western Blot

Figure 6. Effects of ROS scavenger on HO-1 expression in BV2 cells. BV2 cells were pretreated with EBB for 1 h before treatment with 0.2 µg/mL LPS for 24 h. The protein expression of HO-1 was determined

Journal: Life

Article Title: In Vitro Antioxidant and Anti-Neuroinflammatory Effects of Elsholtzia blanda Benth

doi: 10.3390/life15060983

Figure Lengend Snippet: Figure 6. Effects of ROS scavenger on HO-1 expression in BV2 cells. BV2 cells were pretreated with EBB for 1 h before treatment with 0.2 µg/mL LPS for 24 h. The protein expression of HO-1 was determined

Article Snippet: BV2 microglia were purchased from AcceGen (Fairfield, NJ, USA).

Techniques: Expressing